Signalling

Part:BBa_K150000:Design

Designed by: Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne   Group: iGEM08_Heidelberg   (2008-10-21)


3OC6HSL Sender Controlled by constitutive medium strong Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sender part BBa_F1610 was cloned behind the constitutive promoter BBa_J23107 into the plasmid BBa_J61002.


Source

The luxI gene is derived from Vibrio fischerii.

References